Aspirin-Induced Alterations in Male Reproductive Function: Evidence from A Murine Model

Nagia Alghoul; Samira Sasi; Eman Ashwige; Baraa Almokhtar; Ragil  prastiya; Amung Saputro; Trilas Sardjito

doi:10.54361/ajmas.269514

Authors

Nagia Alghoul Libyan Center of Biotechnology Research, Tripoli, Libya https://orcid.org/0000-0002-4850-5046
Samira Sasi Developmental Biology Division. Zoology Department, Faculty of Science, University of Tripoli, Tripoli, Libya https://orcid.org/0000-0002-9436-8548
Eman Ashwige Developmental Biology Division. Zoology Department, Faculty of Science, University of Tripoli, Tripoli, Libya https://orcid.org/0009-0009-4390-5161
Baraa Almokhtar Developmental Biology Division. Zoology Department, Faculty of Science, University of Tripoli, Tripoli, Libya https://orcid.org/0000-0001-9121-9355
Ragil prastiya Faculty of Health, Medicine and Life Sciences (FIKKIA), Universitas Airlangga, Indonesia https://orcid.org/0000-0003-2658-2099
Amung Saputro Faculty of Health, Medicine and Life Sciences (FIKKIA), Universitas Airlangga, Indonesia https://orcid.org/0000-0001-8132-547X
Trilas Sardjito Faculty of Health, Medicine and Life Sciences (FIKKIA), Universitas Airlangga, Indonesia https://orcid.org/0000-0002-2956-7145

DOI:

https://doi.org/10.54361/ajmas.269514

Keywords:

Aspirin, Mice, Testes, Sperm quality, Embryo.

Abstract

Aspirin (ASA) is one of the non-steroidal anti-inflammatory drugs; it is used for treating pain, cold, and fever. This study aimed to evaluate the effect of ASA on sperm parameters, testicular tissues, and the embryos in adult male mice. Sixteen male mice were divided into two groups: the control group received distilled water, while the second group was given ASA at a dose of 75 mg/kg BW for 15 days. At the end of the dosing, measurements were taken for body and testicular weights, testes were removed, and sperm parameters were evaluated, as well as a histopathological assessment of the testes was carried out. Three treated males from each group were put with untreated females (1: 2) for mating. The results showed a significant decline in testicular weight, sperm count, motility, and histopathological criteria and Johnsen^'s score, while the percentage of abnormal sperm increased. Histopathological analysis indicated pronounced testicular alterations in the ASA-treated group compared to the control group. Furthermore, a reduction was observed in both the mean body weight of embryos and the average number of live embryos. Aspirin at the dose of 75 mg/kg exerted adversely influences on sperm quality, testicular tissues, and the embryos. Therefore, ASA should be used with caution.